Journal: EMBO reports

Article Title: The cell cycle protein MAD2 facilitates endocytosis of the serotonin transporter in the neuronal soma.

doi: 10.15252/embr.202153408

Figure Lengend Snippet: Figure 1. The serotonin transporter C-terminus harbors a potential MAD2 interaction motif (MIM). MAD2 protein expression in dorsal raphe neurons.

Article Snippet: Two days after transfection, cells were used for co-IP experiments as described above. siRNA transfection HEK-293 cells in different culture formats were transfected with three target-specific siRNAs against human MAD2 (sc-35837, Santa Cruz Biotechnology) or with siRNA against luciferase (Dharmacon) as a control.

Techniques: Expressing

Journal: PloS one

Article Title: Taxifolin enhances andrographolide-induced mitotic arrest and apoptosis in human prostate cancer cells via spindle assembly checkpoint activation.

doi: 10.1371/journal.pone.0054577

Figure Lengend Snippet: Figure 11. The effect of MAD2 knockdown on the mitotic index of DU145 cells treated with Andro and/or Taxi. Cells were either treated with Andro and/or Taxi for 24 h or transfected with the negative control siRNA or MAD2 specific siRNA for 24 h and later treated with Andro and/or Taxi for additional 24 h. Then cells were fixed and stained with anti-phospho-histone H3 (Ser10) antibody, FITC-conjugated secondary antibody and PI, and analyzed by flow cytometry. Mitotic index is expressed as mean 6 s.d. * indicates significant difference between two groups, *p,0.01. doi:10.1371/journal.pone.0054577.g011

Article Snippet: RNAi depletion of MAD2 DU145 cells were seeded in 60 mm plates to provide a confluency of 50–70% in 24 h. The cells were transfected with either one of the two Mad2L1 siRNA (Origene, MD, USA) or the control siRNA in the presence of siTran transfection reagent (Origene) in Opti-MEM medium (Invitrogen, CA, USA) for 24 h. The transfected cells were rinsed one time with PBS and incubated with DMEM (Invitrogen, CA, USA) medium for 24 h and were further incubated for another 24 h in medium containing 20 mM Andro and 100 mM Taxi.

Techniques: Knockdown, Transfection, Negative Control, Staining, Flow Cytometry